Single-cell proteomics captures the Proteome Heterogeneity in Human iPSC-Derived Cardiomyocytes and Adult Cardiomyocytes.
Project description (from MassIVE): Human induced pluripotent stem cell (IPSC)-derived cardiomyocytes (iCMs) have become important tools to model cardiovascular diseases and drug toxicology. Despite suggested transcriptomic heterogeneity in both iPSC and iCMs, the cellular proteome heterogeneity is poorly understood. Using cutting-edge single cell proteomics, we quantify the maturation from IPSC to iCMs and observed two distinct populations of iCMs with different metabolism, which recapitulates the single adult cardiomyocyte proteome populations albeit less mature.
The two DIA-NN report files are downloaded from the MassIVE dataset MSV000094438 (doi:10.25345/C5T727S7Q) are redistruted here are:
Adult cardiomyocyte (aCMs): 299 cells
iPSC-derived cardiomyocytes (iCMs): 2184 cells
Dataset license: CC0 1.0 Universal (CC0 1.0)
Ai, Lizhuo, Aleksandra Binek, Vladimir Zhemkov, Jae Hyung Cho, Ali Haghani, Simion Kreimer, Edo Israely, et al. 2025. “Single Cell Proteomics Reveals Specific Cellular Subtypes in Cardiomyocytes Derived from Human iPSCs and Adult Hearts.” Mol. Cell. Proteomics, no. 100910 (January): 100910. https://doi.org/10.1016/j.mcpro.2025.100910.