These data were generated based on publicly available DIA benchmarking dataset from Gotti et al. (2021). A subset of raw data, containing "overlapped" in the File.Name were searched using the DIA-NN software, and the resulting report.tsv (here labelled as 'benchmarkingDIA.tsv') is provided.
The dataset contains 8 conditions containing a mix of E.coli and Universal Standard Protein-1 (UPS1) peptides. Per 1 ug of E.coli protein (equal in all samples), UPS1 proteins are diluted to final concentration of 50, 25, 10, 5, 2.5, 1, 0.25 and 0.1 fmol.
Each sample was prepared in 3 replicates, so altogether there are 24 samples in the dataset.
Gotti C, Roux-Dalvai F, Joly-Beauparlant C, Mangnier L, Leclercq M, Droit A. Extensive and Accurate Benchmarking of DIA Acquisition Methods and Software Tools Using a Complex Proteomic Standard. J Proteome Res. 2021 Oct 1;20(10):4801-4814. doi: 10.1021/acs.jproteome.1c00490. Epub 2021 Sep 2. PMID: 34472865.