The sciex mzML files represent profile-mode LC-MS data of pooled human serum samples (the same pool being measured). The samples were analyzed by ultra high-performance liquid chromatography (UHPLC; Agilent 1290) coupled to a Q-TOF mass spectrometer (TripleTOF 5600+ AB Sciex). The chromatographic separation was based in hydrophilic interaction liquid chromatography (HILIC) and performed using an Waters Acquity BEH Amide, 100 x 2.1 mm column.
The mass spectrometer was operated in full scan mode in the mass range from 50 to 1000 m/z and with an accumulation time of 250 ms. The files represent a subset of spectra/scans from m/z 105 to 134 and from retention time 0 to 260 seconds. The files were generated in the same LC-MS run, but from different injections. Details on the individual files are provided below.
Files:
20171016_POOL_POS_1_105-134.mzML: profile-mode LC-MS data of pooled human serum samples. Injection index: 1.
20171016_POOL_POS_3_105-134.mzML: profile-mode LC-MS data of pooled human serum samples. Injection index: 19.